Non-Mammalian Flow Cytometry

Professor David Galbraith – University of Arizona, United States
David Galbraith, MA, PhD, is a Professor in the School of Plant Sciences and the BIO5 Institute for Collaborative Bioresearch at the University of Arizona. He is an adjunct faculty member in the Department of Biomedical Engineering, and a member of the Arizona Cancer Center. He has extensive expertise in biological instrumentation, instrument development, and biological applications of these instruments. His is recognized as a pioneer in the field of flow cytometry and cell sorting particularly as applied to plants. Dr. Galbraith’s interests include DNA and protein microarrays and NextGeneration DNA sequencing for genotyping and expression analysis, flow cytometry and cell sorting, confocal and multiphoton imaging, GFP expression and subcellular targeting, and single cell/single nucleus genomics and transcriptomics. He applies his technologies to address problems and disease states in plant and animal biology. He produces and works with genetically engineered plants and animals. He has served on the Scientific Advisory Board for Accuri, and also served as a Consultant to Beckman Coulter, Agrigenetics, Monsanto, Ventana Medical Systems, Stratagene, BASF, Roche, High Throughput Genomics, Driscoll’s, and BioRad. He was elected Fellow of the American Association for Advancement of Science in 2003, and was elected Secretary of the International Society for Advancement of Cytometry in 2016. He has published over 200 articles in scholarly journals, and these have been cited (Google Scholar) over 19,500 times. He has given 353 invited presentations at scientific meetings, to universities and institutes world-wide, and for industrial clients.

Non-Mammalian Flow Cytometry

Dr. Allison McInnes – Queensland University of Technology, Australia
Allison McInnes, PhD is a Senior Research Analyst at the Centre for Microbiome Research at the Queensland University of Technology in Brisbane. Her PhD and postdoctoral research focused on the role of phytoplankton and microbes in marine biogeochemical cycles. She developed novel flow cytometric methods to probe specific functions of the marine community including, simultaneous carbon and nitrogen fixation measurements using CARD-FISH, stress response to temperature using ROS probes, silicification of diatoms using PDMPO, as well as standard methods for quantification of the entire community from viruses to eukaryotes. In her current role her focus is on developing and optimising methods to isolate, cultivate, and sort for multi-omics, microbes from various host-associated environments (coral, soil, permafrost, and faecal).

High-Dimensional Full-Spectrum Panel Design and Optimisation

Dr. Laura Ferrer Font – Malaghan Institute of Medical Research, New Zealand
After finishing her PhD in Barcelona Autonomous University, she took a Postdoctoral position in the Cancer Immunotherapies Programme at the Malaghan Institute of Medical Research, and it was she became involved with spectral flow cytometry and was totally captivated by the potential of this technology. In 2019, she joined the Hugh Green Cytometry Centre as a High Dimensional Cytometry Specialist and in 2020 as the Deputy Manager. She has been involved in many different clinical and fundamental research projects, she has published several protocols about the use of spectral flow cytometry and have delivered numerous workshops. She is very passionate about science education.

Extracellular Vesicles and Flow Cytometry

Professor Georges Emile Raymond Grau – Chair of Vascular Immunology, Department of Pathology, School of Medical Sciences, Faculty of Medicine & Health, University of Sydney, Australia
Professor Georges Grau, MD (University of Liège) and Privat-Docent (University of Genève), is the Chair of Vascular Immunology at the University of Sydney since 2006. His research in immunopathological mechanisms of infectious diseases focuses on cytokines and the microvascular endothelium.

His 386 papers have been cited over 35,000 times and his h-index is 98. Since 2015 he serves as Discipline Leader (Pathology), Marie Bashir Institute, and in 2017 he was elected President of the Australian-New Zealand Microcirculation Society (ANZMS).

Microfluidics

Dr. Sajad Razavi Bazaz – University of Technology Sydney, Australia
Sajad Razavi Bazaz studied Biomedical Engineering at University of Technology Sydney, Sydney, Australia. His PhD topic mainly revolved around the topic of 3D printed microfluidic devices, where he was able to develop a new patented additive manufacturing method for the fabrication of microfluidic devices and validate it through multiple microfluidic applications, ranging from sample processing to particles/cell sorting, cytometry applications, and intracellular delivery studies. Based on the techniques he developed and the knowledge he gained during his PhD, his team received 20,000 AUD$ in research funding from UTS Faculty of Engineering and IT Deep Tech Challenge 2019; later on, they awarded “Most Innovative Prototype Award 2019” and “People’s Choice Award 2019” at UTS Techcelerator program. Later in 2020, Sajad was awarded the prestigious award of Royal Society of NSW Scholarships as the best researcher of New South Wales. Upon the knowledge he gained so far, Sajad is investigating on miniaturization of current cytometry platforms and is working on the development of microfluidic-based flow cytometry devices.

High-Dimensional Full-Spectrum Panel Design and Optimisation

Sam Small – Malagan Institute of Medical Research, New Zealand
Sam Small is a senior staff scientist within the Hugh Green Cytometry Centre at the Malaghan Institute of Medical Research. Her focuses in this role are centred around supporting researchers in all things flow cytometry related, from training a range of users from beginner through to advanced, to panel design, data analysis and troubleshooting, cell sorting, and other ongoing support. She is passionate about ensuring high standards are maintained when producing flow cytometry data, striving to improve researcher capability through training and education.

Introduction to Cytometry Data Analysis in R

High Dimensional Data Analysis: Introduction

High Dimensional Data Analysis: Advanced

Dr. Thomas Ashhurst – Sydney Cytometry Core Research Facility, University of Sydney and Centenary Institute, Australia
Dr. Thomas Ashhurst is a high-dimensional cytometry specialist with the Sydney Cytometry Core Research Facility, and an Honorary Research Fellow with the Infection, Immunity, and Inflammation Theme, School of Medical Sciences, at The University of Sydney. In these roles he provides advanced training and consultation for users of high-dimensional cytometry, imaging, and single-cell sequencing technologies. He also leads a multi-disciplinary research initiative to develop a range of high-dimensional single-cell and imaging methods, as well as advanced computational analysis approaches, and apply them to comprehensively map dynamic immune responses over time and space using a ‘systems immunology’ approach. His research is particularly focused on the study of inflammation and infectious diseases, including emerging pathogens such as SARS-CoV-2/COVID-19, Zika virus encephalitis, and West Nile virus encephalitis.

Shared Resource Laboratory Biosafety

Associate Professor Evan Jellison – University of Connecticut Health Center, United States
Evan earned his doctoral degree from the University of Massachusetts Medical School, where he studied immune responses to viral infections. He then entered into postdoctoral training at UCONN School of Medicine studying immune tolerance in memory T cells.

Throughout his training, flow cytometry has played a vital role in Evan’s research and he gained substantial expertise in the technology. This ultimately resulted in him being hired to continue on at UCONN School of Medicine as a professor and director of flow cytometry. In this role, Evan continues to play an important role in nearly every aspect of research at the medical school, while training and educating students, fellows, and even the occasional P.I.

Recently, Evan has migrated some of his expertise from the SRL into the clinical lab where he is the supervisor for clinical flow cytometry at UCONN’s John Dempsey Hospital. Evan is an active member of his regional flow cytometry societies (Metroflow and New England Cytometry) and maintains an active membership role with ISAC, serving on both the ISAC Biosafety Committee and as co-chair of the Flow Cytometry Content sub-committee.

Shared Resource Laboratory Biosafety

Avrill Aspland – Sydney Cytometry Core Research Facility, University of Sydney and Centenary Institute, Australia
Avrill is Operations Coordinator for Sydney Cytometry and key to her role is the management of biosafety processes. She provides key technical expertise to the application of biosafety control measures, collaborating closely with safety officers and researchers. Through this collaboration and oversight of the safety framework she facilitates efficient access to technologies, while maintaining a safe space for all staff and users. Avrill is a member of the ISAC Biosafety Committee.

Introduction to Cytometry Data Analysis in R

High Dimensional Data Analysis: Introduction

High Dimensional Data Analysis: Advanced

Dr. Felix Marsh-Wakefield, Centenary Institute, Australia
Felix Marsh-Wakefield is a post-doctoral researcher part of the Liver Injury & Cancer (Centenary Institute) and Human Cancer & Viral Immunology Laboratory (University of Sydney). He is an immunologist interested in investigating the role of various immune cells in a range of diseases, including hepatocellular carcinoma and multiple sclerosis. This primarily involves bioinformatics to assist in the analysis of high dimensional data, including that of imaging mass cytometry. He is part of the Marylou Ingram Scholarship Program run by the International Society for the Advancement of Cytometry (ISAC).