Panel design and analysis strategies to characterize dim signals in highly autofluorescent samples with Alexis Gonzalez & Kate Pilkington
Sunday , Nov 20
Autofluorescence. It is, by its very nature, endogenous in every biological sample we analyse. In conventional flow cytometry, the predominant management strategy has been to make panel designs that completely avoid channels with high autofluorescence, while it has been somewhat effective, it has some significant limitations. With full spectrum profiling we are now learning the finer details about the autofluorescent characteristics of our samples, giving us the opportunity to elegantly manage this complex issue in our flow cytometry data with advanced data analysis methods and better yet, improved panel design.
What You’ll Learn:
In this workshop we will discuss ways in which you can manage your autofluorescence in order to maximise the resolution of marker expression that you can obtain from your data. Including, strategies to analyse and extract complex autofluorescence, with guidelines about when and why this might be necessary. However, it is far easier if we plan our panel design to optimise the marker and fluorochrome assignment in a way that accommodates the finer details of your autofluorescence, therefore, we will also discuss panel design strategies to optimise your panels to fit your sample autofluorescence.